modification of 10 cgy neutron or gamma-rays induced chromosomal damages by hyperthermia: an in vitro study

نویسندگان

d. fatehi department of medical physics, faculty of medicine, shahrekord university of medical sciences, shahrekord, iran

h. mozdarani department of medical genetics, faculty of medical sciences, tarbiat modares university,tehran, iran

چکیده

background: to evaluate the effects of hyperthermia (ht) on the frequency of chromosomal aberrations induced by a low dose of neutron or γ-rays in human peripheral blood lymphocytes. materials and methods: blood samples were exposed to ht (41.5°c for 30 and 60min, 43°c for 15 and 30min), 10 cgy neutron or γ-rays, ht + neutron/γ, and neutron/γ + ht. after standard cell culture, harvesting, fixation and staining, the chromosomal damages were scored in metaphase plates. results: ht alone at 41.5°c did not induce chromatid or chromosome aberrations, however, the frequency of damages was significantly higher at 43°c (p<0.05). furthermore, the chromosomal damages was significantly different when cells were irradiated with neutron or γ-rays alone (p<0.01). ht 1 hr post neutron/γ irradiation significantly induced higher chromosome damages in comparison to ht 1 hr before irradiation (p<0.05). the chromosomal damages were remarkably higher when cells were irradiated with neutron then heated at 43°c for 30 min. conclusion: since increasing frequency of chromosome damages increases probability of cell death, application of ht after neutron irradiation (instead of x– or γ- rays) might be considered as a procedure for cells killing in radiotherapy. iran. j. radiat. res., 2009 7 (2): 69-77

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عنوان ژورنال:
iranian journal of radiation research

جلد ۷، شماره ۲، صفحات ۶۹-۷۷

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